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DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems automated and non-automated customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. The gel was run at V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.
Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. Click here to view the general protocols. Publishing research using ab?
Please let us know so that we can cite the reference in this datasheet. Can you please send me the amino acid sequence of the peptide used for immunization to make these antibodies? Thank you for contacting us. While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets: ab Synthetic peptide based on the Human sequence. The antibody epitope is located in loop 3 of TIMP2.
The epitope is located in loop 1 of TIMP2. I hope this helps, please let me know if you need any additional information or assistance. Thank you for your offer. As for the IP, I am not sure me or my colleagues plan to do it in time for the expiry date of the offer you mention. But I won't totally rule it out either ; Anyhow, thank you very much for your help. Just one last question. Could you please let me know by when I should expect the delivery for the antibody? I would, of course, like to have it as soon as possible, since no one will be in the lab from the 6th to the 9th of April.
Hope to hear from you soon! Many Thanks and Regards,. Thank you for your response. This is to confirm thatI have placed a new order for you - for one vial ofab as a free of charge replacement and the new order number is and the item will be shipped next Tuesday 10th April after Easter break.
I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product. Thank you for your reply. Plus I donot know if a testing discount was offered on the product for IP, since I remember paying the full amount for the antibody. So everything was done according to the datasheet. I have to say this is the first time I'm experiencing problems with an Abcam antibody, and was hence wondering what we could do about it!
Please do let me know! The discount code has been issued for you for testing this antibody in IP. I am forwarding my colleague's e-mail below so please read the discount offer. If you have any questions, please do not hesitate to contact us again.
Nevertheless, I can certainly offer you a new vial feel of charge replacement for you if this antibody does no longer work in Western blot and IHC. Let me know if you wish to get a new vial. Previous e-mail: Thank you for contacting us. Your order has the reference number and you should receive it as discussed by 06 February. I am very pleased to hear you would like to accept our offer and test ab in IP. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1 Call to place your order and mention the code to our customer service department; 2 Include the code in your fax order; 3 Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. It worked for the first couple of times, but after that, the antibody seems to have lost activity, since I cannot detect it anymore, on WBs or even on IHC. We have been regular users of Abcam products and never have we experienced any sort of troubles with your products.
They always worked perfectly well. But in this case, I just wanted to report this discrepancy to you, and ask if you could maybe replace the antibody with another vial but, probably from another batch or so. Also, this antibody is quite important for my project, and I would like to have it as soon as possible. If theres any queries, you may please get in touch with me at the numbers listed below.
Please do let me know what can be done. Hoping to hear from you soon! Thank you for your contact regarding ab I am very sorry to hear that you are having problem with this antibody. I understand from your e-mail this antibody has been used in Western blot and IHC application and it worked initially but failed to work later on.
According to our record, testing discount code was offered to you to test one vial ab in immunoprecipitation IP. Have you had a chance to use this product in IP? Would you be so kind to explain how the antibody was stored after arrival? I look forward to hearing from you and hope to solve this problem as soon as possible. Is the Western blot information in the datasheet for this antibody based on native or denaturing conditions?
Send me a copy of this email. I agree to the terms and conditions. Peptide available as ab Associated products. IGFBP7 peptide ab Our Abpromise guarantee covers the use of ab in the following tested applications. Stimulates prostacyclin PGI2 production. Stimulates cell adhesion. Contains 1 Ig-like C2-type immunoglobulin-like domain. Contains 1 Kazal-like domain.
Performed under reducing conditions. DAPI was used to stain the cell nuclei blue at a concentration of 1. Immunohistochemistry protocols. Datasheets and documents. Submit a review Submit a question. Abreviews Abreviews. Read More. Abcam user community Verified customer Submitted Jan 08 Abcam user community Verified customer Submitted Oct 29 Abcam Scientific Support. Many Thanks and Regards, Read More. Abcam user community Verified customer Submitted Apr 16 Abcam user community Verified customer Submitted Jan 14 Get resources and offers direct to your inbox Sign up.
Detects a band of approximately 35 kDa predicted molecular weight: 29 kDa. Use at an assay dependent concentration.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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