Phytochemical study of Senna alata using two methodologies. Rodrigues, I. I ; Souza Filho, A. II ; Ferreira, F.

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Phytochemical study of Senna alata using two methodologies. Rodrigues, I. I ; Souza Filho, A. II ; Ferreira, F. In this study, two different methodologies are compared to determine the main classes of potential allelochemicals of different fractions stems, flowers, leaves, roots, seeds and pods of S. The plant material was dried and submitted to exhaustive extraction with hydromethanolic solvent to obtain the crude extracts, with a small part being solubilized in methanol to obtain the test solutions.

The following methods were used: thin-layer chromatography TLC , to determine the qualitative chromatographic profile, and preliminary detection tests of the distinct chemical constituents, based on their extraction with appropriate solvents and application of color testing. The results of both methods showed few similarities, with the TLC being the simplest, most inexpensive, fastest, and most appropriate for preliminary analysis of plant-derived chemical compounds, despite being a qualitative method.

This method was more sensitive for flavonoid detection, although the Bouchardat reactive was more sensitive to alkaloid detection than the Dragendorff. The study showed the high diversity of the chemical compounds present in Senna alata , justifying its extensive use in popular medicine and even indicating its potential allelopathic use. Keywords: Allelochemicals, Thin-layer chromatography, Phytochemistry, Leguminosae.

Coleta e processamento do material vegetal. O plantio ocorreu em fevereiro de Preparo dos extratos brutos. O resultado da cromatografia em camada delgada pode ser observado na Tabela 2. Segundo Gianvechio et al. Stimulation of growth and development of Celosia argentea L. Bianthraquinones from the seeds of Senna multiglandulosa. Phytochemistry , v.

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Allelopathy: state of the science. The science of allelopathy. RICE, E. Orlando: Academic Press, Estudio fitoquimico y atctividad antialimentaria de Senna stipulaceae. Plant drug analysis - A thin layer cromatography atlas. Berlim: Springer, All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

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